Figure 1.

Interactions between CD40B cells and CTLs are short-lived. Cyclin D1-specific T cells were embedded in 3D collagen matrices together with different APCs: resting B cell (B cell), CD40B cells (CD40B), immature (DCimm) and mature (DCmat) DCs. APCs were pulsed with 10 µg/mL of the peptide cyclinD1_228 were indicated. Cell movements were recorded by time-lapse video microscopy and the duration of individual T cell-APC contacts was analyzed. (A) Each dot represents one contact. Bars represent the median. *p < 0.001. Data are pooled from 34 films from 9 independent experiments. (B) The percentage of cell contacts that last longer than 45 min of all contacts are shown. *p < 0.002.