Figure 2.

CD40B cells express crucial molecules for homing in to secondary lymphoid organs. Purified B cells were stimulated with tmuCD40L HeLa in the presence of rmIL-4. After 3 d, cells were stimulated with fresh CD40L and rmIL-4. (A and B) The B cells were phenotyped for the indicated molecules before stimulation with CD40L and (C) on day 7 of culture. Expression on cells gated for B220⁺ CD3⁻ is presented in the histograms. Shaded histograms represent staining with isotype matched control antibodies; black lines staining with specific antibody. One representative experiment out of 9 is shown.