Figure 5.

CD40B cells migrate to spleens and lymph nodes in healthy and tumor-bearing mice. 5–10 × 10⁶ CD40B cells from luciferase⁺ mice (A and C) or GFP⁺ mice (B) were injected into C57BL/6 mice subcutaneously (s.c.) or intravenously (i.v.). 12 hours (12 h), 36 hours (36 h) or 5 days (5 d) after injection, mice were analyzed for the presence of the CD40B cells either by imaging in the IVIS 200 system in healthy mice (A) or EL4 tumor-bearing mice (C). Cy3 stained to detect all B220+ B cells (red); GFP⁺ CD40B cells (green) by confocal microscopy (B, upper panel). Representative pictures out of 3 experiments are shown. The number of GFP+ CD40B cells was determined at the indicated time points (B, lower panel). Mean values from one representative experiment are shown; n = 2 mice per time point and condition from each independent experiment.