Skip to main content
. 2015 May 27;4(9):e1027471. doi: 10.1080/2162402X.2015.1027471

Figure 6.

Figure 6.

TRX confers Treg enhanced tolerance to oxidative stress in melanoma. (A) Reactive oxygen species (ROS) level in B16 tumor-bearing mice tumor interstitial fluid (n = 3) determined by Acridan Lumigen PS-3 reagent. (B) Cell apoptosis was determined by propidium iodide (PI) and AnnexinV staining and cytofluorimetric analysis of freshly isolated regulatory T cell (Tregs) and effector T (Teff) cells cultured in the presence of tumor interstitial fluid (n = 3). (C-D) Cell apoptosis (PI and AnnexinV staining) of freshly isolated Tregs cultured in the presence of tumor interstitial fluid plus either (C) 10 ng/mL reduced thioredoxin (rTRX; n = 3) or (D) 1 mg/mL anti-TRX antibody (n = 3). (E) FACS analysis of Teff and Treg apoptosis in isolated cells from B16 tumor-bearing mice per 200 mg tumor sample (n = 5). (F) FACS analysis of Treg apoptosis in isolated cells from TRX-overexpressing (OE) and TRX knockdown (KD) B16 tumor-bearing mice per 200 mg tumor sample (n = 5). Statistical analysis was performed by Student's t-test; *P < 0.05.