Figure 2.

Immunization with OVA-LeB and OVA-aDC-SIGN increases T cell priming in vivo. hSIGN mice were immunized s.c. with either OVA-LeB, OVA-aDC-SIGN or native OVA mixed with anti-CD40 using a prime-boost protocol. Spleens were examined for the frequency of OVA-specific CD8⁺ and CD4⁺ effector T cells 1 week after boosting. (A–C) OVA-aDC-SIGN and OVA-LeB augmented generation of poly-functional effector CD8⁺ T cells, as revealed by higher frequencies of IFNγ- and TNF-double and IFNγ-single producing CD8⁺ T cells after o/n re-stimulation with OVA257-264. (D) Targeting antigen to DC-SIGN also enhanced induction of CD4⁺ effector T cells as shown by intra-cellular IFNγ-staining after 2 d restimulation with OVA262-276. Dashed lines represent frequencies of cytokine-secreting T cells in naive mice. n = 5 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001. Results shown are representative of two independent experiments.