Figure 1.

AdCMVdelta24 increases infiltration of leukocytes into tumor-bearing brains. Flow cytometry analysis of CD45 ^high leukocytes, CD45 ^high CD11b⁺ monocytes, CD45^high CD11b⁺ F4/80⁺ macrophages, and CD3⁻ NK1.1⁺ NK cells isolated from the brain was performed. Changes of the frequency (top panel, Leukocytes (A); Macrophages (B); NK cells (C)) and the total number (bottom panel, Leukocytes (D); Monocytes (E); Macrophages (F)) of the cells in mice brains are presented. C57BL/6 mice bearing 5 d-established intracranial GL261 tumor were injected intratumorally with AdCMVdelta24 or control PBS. Three days later, the flow cytometry analysis was performed. Data are presented as mean ± SEM in three or four mice for each treatment group. *p < 0.05, **p < 0.01 and ***p < 0.001 (unpaired, two-tailed Student's t-test). CD45Hi, CD45^high.