Figure 4.

Intratumoral injection of AdCMVdelta24 modulates tumor microenvironment toward immune stimulatory state. C57BL/6 mice bearing 5 d intracranial GL261 glioma were injected intratumorally with AdCMVdelta24 virus or control PBS. Two weeks after viral treatment, flow cytometry analysis was performed on brains and cLNs leukocytes. (A–D) An increased percentage of CD4⁺ (A), CD4⁺IFNγ⁺ (B), CD8⁺ (C), and CD8⁺IFNγ⁺ (D) T cells in brains was observed. (E–G) An increased ratio of CD4⁺IFNγ⁺ to CD4⁺Foxp3⁺T cells (E), and CD4⁺RORγt⁺ to CD4⁺Foxp3⁺ (G) T cells was observed in brains, but not in cLNs (F, H). Data are presented as mean ±SEM in four to five mice for each treatment group. *p < 0.05, **p < 0.01, ***p < 0.001, and n.s., not significant (unpaired, two-tailed Student's t-test).