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. 2015 Apr 2;4(9):e1027468. doi: 10.1080/2162402X.2015.1027468

Figure 1.

Figure 1.

NK cells from Tlr3−/− mice display defective cytokine production. NK cells were purified from the spleens of wild-type (WT) or Toll-like receptor 3 null (Tlr3−/−) mice and cultured together with variable concentration of recombinant (r) IL-12 with constant 50 ng/mL rIL-18 or with variable concentration of rIL-15 with constant concentration of 1 ng/mL rIL/12 (A, D, and E), with 500 pg/mL of rIL-12 combined with 50 pg/mL of rIL-18 (C, F) or with 10 ng/mL of PMA together with 1 μg/mL of ionomycin (B). After 16 to 20 h, supernatants were collected and IFNγ (A, B), MIP-1α (E) or MIP-β, RANTES, IL-6 and GM-CSF (F) production was assessed by CBA. Alternatively, cells were stained and NK cell activation (C) or IFNγ production (D) were analyzed by immunofluorescence staining and cytofluorimetric analysis. Data shown are representative of at least 2 independent experiments and presented as mean ± SD of 3 experimental replicates. The Mann-Whitney test was used to compare differences between WT and TLR3-/- NK cells; **P < 0.01; ***P < 0.001).