Skip to main content
. 2015 Sep 15;5:14066. doi: 10.1038/srep14066

Figure 1. Stability, reproducibility, and specificity of the unfixed cell sensor surface.

Figure 1

Cell coverage on the sensor surface was evaluated before (A) and after (B) QCM measurements by staining the nuclei of SKOV-3 cells with Hoechst 33342 and visualizing under a fluorescent microscope (Olympus BX53). (C) Reproducibility of 5 cycles of the interaction between WGA (50 μg/mL) and unfixed SKOV-3 cells on sensor surface with regeneration step (2 injections of 300 mM GlcNAc) in between. (D) The bindings of WGA-FITC (red line) and Alexa Fluor® 488 conjugated GS-II (blue line) with SKOV-3 cells were evaluate by QCM measurements and microscopic evaluation. Fluorescent images of nuclei (in blue) and lectin (in green) were taken before (a) and after (b) WGA-FITC; (d) Alexa Fluor® 488 conjugated GS-II injection of lectin, as well as after regeneration (c). The scale bars stand for 40 μm.