Table 1. Relative percent analyses of different protein bands.
Protein production | TBH(Hpl/Hpl) | WT(−/−) |
---|---|---|
Fib-H/fibroin (w/w) (%) | 71.01 | 91.86 |
HPL/fibroin (w/w) (%) | 18.85 | 0 |
HPL/cocoon shell (w/w) (%) | 15.46 | 0 |
The silk fibroin was approximately 82% of the cocoon shell silk protein in the experimental varietiy, and the molar ratio of fibroin is Fib-H (350 kDa) : Fib-L (26 kDa) : P25 (30 kDa) (w:w:w) = 6:6:114,44. The molecular mass of HPL protein was approximately 120 kDa. Using Fib-L as an internal control, HPL radio of fibroin in TBH was calculated according to the following formula: HPL(%) = W(HPL) ÷ [W(HPL) + W(Fib − H) + W(Fib − L) + W(P25) × 100]. In the formula, = molecular weight (M)×mole number (n). SDS-PAGE bands were quantified by a Gel-PRO ANALYZER.