Table 1. Relative percent analyses of different protein bands.

Protein production	TBH(Hpl/Hpl)	WT(−/−)
Fib-H/fibroin (w/w) (%)	71.01	91.86
HPL/fibroin (w/w) (%)	18.85	0
HPL/cocoon shell (w/w) (%)	15.46	0

The silk fibroin was approximately 82% of the cocoon shell silk protein in the experimental varietiy, and the molar ratio of fibroin is Fib-H (350 kDa) : Fib-L (26 kDa) : P25 (30 kDa) (w:w:w) = 6:6:1¹⁴,⁴⁴. The molecular mass of HPL protein was approximately 120 kDa. Using Fib-L as an internal control, HPL radio of fibroin in TBH was calculated according to the following formula: HPL(%) = W(HPL) ÷ [W(HPL) + W(Fib − H) + W(Fib − L) + W(P25) × 100]. In the formula,  = molecular weight (M)×mole number (n). SDS-PAGE bands were quantified by a Gel-PRO ANALYZER.