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. 2015 Sep 15;5:14041. doi: 10.1038/srep14041

Figure 5. Specific re-expression of GHSR in β-cells of GHSR-null/Ins-Cre mice.

Figure 5

(A) RT-PCR analysis showed that the Ghsr mRNA was expressed in isolated islets and pituitary (Pit.) from wild-type mice (Wild), but not detectable in those from GHSR-null mice (Null). GHSR-null/Ins-Cre mice (Null/Cre) exhibited Ghsr mRNA expression in islets but not in pituitary. (B) In isolated islets from GHSR-null/Ins-Cre mice, ghrelin (10 nM) markedly attenuated the glucose (8.3 mM)-induced insulin release. n = 8 (tubes of batch incubation). (C) In the presence of IBMX (500 μM), glucose (8.3 mM)-induced cAMP production in isolated islets from GHSR-null/Ins-Cre mice was inhibited by ghrelin (n = 6). (D and E) In single β-cells from GHSR-null/Ins-Cre mice, ghrelin suppressed glucose (8.3 mM)-induced [Ca2+]i increases. n = 148–214 (single β-cells). *P < 0.05, **P < 0.01.