Figure 1.

Conceptual comparison of MSI (top) and heat mapping (bottom) experiments as they scan a sample from left to right. Imaging experiments have a continuous flow of data, while heat maps are of specified locations. However, for each specific location in the heat map, there is chromatographic separation and UV/vis data associated with it. In this hypothetical example, the color scale indicates the relative amount of signal detected for the given analytes.¹³