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Droplet–LMJ–SSP coupled to UPLC–PDA–HRMS–MS/MS was used to sample fungus G100, thereby generating (A) the total ion chromatogram and (B) the UV/vis (190–500 nm) chromatogram (0.08 min delay between PDA and MS). At 4.35 min, (C) the HRMS spectrum, (D) the HCD fragmentation pattern, and (E) the UV/vis spectrum can be observed, all corresponding to 1. The mass accuracy of 1 was −0.6 ppm (225.1120 observed vs 225.1121 calculated for [C12H16O4 + H]+).
