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. 2015 Sep 14;6:8205. doi: 10.1038/ncomms9205

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(a,b) Schematic representation of mutated zPIP5K1A residues. Green marks those residues, when mutated, causing insolubility. Deep red and blue mark those critical for DIX binding. The rest do not affect the binding. (b) 90° Rotation of a with the membrane interaction interface at the back. (c) Interaction of recombinant zPIP5K1A and its mutants with recombinant DIX. GST-pull-down was performed with His-tagged zPIP5K1A proteins and GST-tagged DIX with GST as a control. The proteins were detected by western blotting. WT, wild type.