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. 2015 Sep 14;6:8266. doi: 10.1038/ncomms9266

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(a) Immunoblot analysis of PU.1, IRF4, BATF, STAT6, and p-STAT6 in WT naive CD4⁺ T cells activated for 1 to 3 days under iTreg-polarizing conditions with DTA-1 or control IgG. Data shown are representative of two independent experiments. (b) Induction of Foxp3⁺ T cells and IL-9+ T cells from WT B6, Stat6^−/−, Spi1^−/−, Irf4^f/fCd4-Cre, and Batf ^−/− CD4⁺ T cells activated under iTreg conditions for 3 days with or without GITR ligation. Numbers in the quadrants indicate the percentage of cells. Data are representative of five independent experiments. (c) Immunoblot analysis of STAT6 and p-STAT6 in WT B6 and p50^−/− naive CD4⁺ T cells activated for 1 to 2 days under iTreg-polarizing conditions with DTA-1 or Ctrl IgG. Data are representative of two independent experiments.