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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Virol Methods. 2014 Aug 4;208:66–78. doi: 10.1016/j.jviromet.2014.07.030

Table 3.

Comparison of antigen reactivity in MAC ELISA of BPL-treated SLEV and CHIKV: with or without hydrolysis treatment

Antigen
Dilution
SLEV - 0.05% BPL

Hydrolysis No Hydrolysis

VSAA NBR VSAA NBR
1:10 0.852 0.050 0.934 0.048
1:20 0.613 0.048 0.744 0.044
1:40 0.401 0.046 0.499 0.047

CHIKV - 0.3% BPL

1:8 1.472 0.156 1.942 0.512
1:16 0.801 0.096 1.329 0.274
1:32 0.477 0.071 0.740 0.145

Abbreviations: VSAA – virus-specific antigen activity; NBR – nonspecific background reactivity.