A–D Treatment with the proteasome inhibitor epoxomicin (epx) is associated with a shift towards reducing conditions in the ER and a shift towards oxidizing conditions in the cytosol in C. elegans (A, B) and HeLa cells (C, D), respectively. The linear range of the ratio-metric analysis is indicated by the values for the fully reduced/oxidized state of the sensors.
E, F Treatment with epoxomicin is associated with a decrease in H2O2 levels in the ER in neuronal and muscle cells in C. elegans (E) and HeLa cells (F).
Data information: The data are presented as means ± SD (A:
P = 3.88 × 10
−14; B:
P = 3 × 10
−16; C:
P = 1.3 × 10
−4; D:
P = 5.02 × 10
−6; E:
P = 1.57 × 10
−14 (neurons) and
P = 5.49 × 10
−12 (muscle); F:
P = 1.2 × 10
−50).The
C. elegans data are based on an analysis of 20 nematodes for each condition and the number of analysed HeLa cells varies between 29 and > 50.
Source data are available online for this figure.