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. 2015 Jul 30;34(18):2334–2349. doi: 10.15252/embj.201591711

Figure 4. Proteasome inhibition is associated with redox perturbation in the ER and cytosol.

Figure 4

  • A–D Treatment with the proteasome inhibitor epoxomicin (epx) is associated with a shift towards reducing conditions in the ER and a shift towards oxidizing conditions in the cytosol in C. elegans (A, B) and HeLa cells (C, D), respectively. The linear range of the ratio-metric analysis is indicated by the values for the fully reduced/oxidized state of the sensors.
  • E, F Treatment with epoxomicin is associated with a decrease in H2O2 levels in the ER in neuronal and muscle cells in C. elegans (E) and HeLa cells (F).
Data information: The data are presented as means ± SD (A: P = 3.88 × 10−14; B: P = 3 × 10−16; C: P = 1.3 × 10−4; D: P = 5.02 × 10−6; E: P = 1.57 × 10−14 (neurons) and P = 5.49 × 10−12 (muscle); F: P = 1.2 × 10−50).The C. elegans data are based on an analysis of 20 nematodes for each condition and the number of analysed HeLa cells varies between 29 and > 50.

Source data are available online for this figure.