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. 2015 Aug 19;34(18):2350–2362. doi: 10.15252/embj.201591382

Figure 1. Pathological examination of Dnmt2-deficient mice.

Figure 1

  1. Giemsa staining of a femoral section from 8-day-old mice showing endochondral ossification defects. Scale bar: 100 μm.
  2. Representative femur sections from 8-day-old wild-type and Dnmt2−/− mice. The staining of perivascular cells (GLS, green) is strongly reduced in Dnmt2−/− bone marrow. Nuclei are counterstained with DAPI. Scale bar: 100 μm.
  3. Quantitative analysis of capillary numbers. GLS-positive capillaries were counted in wild-type (wt) and Dnmt2−/− (D2-) mice, in three independent high-power fields (HPF) per mouse (n = 7).
  4. Quantification of cell numbers in haematopoietic tissues of 8-day-old mice (n = 8).
  5. Representative FACS plots of wild-type and Dnmt2−/− BM stained for Lin− Sca1+ and cKit+ cells.
  6. Quantification of bone marrow LSK cells in wild-type (three independent experiments, each with pooled BM samples from 4 to 6 mice) and Dnmt2−/− bone marrow (three independent experiments, each with pooled BM samples of 5–9 mice).
  7. Restoration of the LSK cell population in adult Dnmt2−/− mice. Frequencies of LSK cells were normalized to the frequencies of LSK cells from the bone marrow of wild-type littermates at the indicated age (2–3 independent experiments, each with pooled bone marrow samples from 3 to 9 mice).
  8. Increased LSK CD34− and reduced LSK CD34+ cell populations in 8-day- and 1-year-old Dnmt2−/− mice compared to wild-type (two independent experiments, each with pooled bone marrow samples from 3–9 mice).
Data information: Data are presented as mean ± SD. Asterisks indicate statistically significant (P < 0.05, t-test) differences. In (G) and (H), asterisks indicate statistically significant (P < 0.05, t-test) differences relative to wild-type (= 1).