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Summary of Experimental Measurements
| EsxB protein | WT-EsxB | WT-EsxB-His6 | WT-EsxB Y65F | WT-EsxB P67A |
|---|---|---|---|---|
| Dimer form in crystal | AP | BU | BU | AP |
| Recognizable quaternary assembly in crystal | Dimer | Dimer/tetramer | Dimer/tetramer | Dimer |
| Apparent oligomer in size-exclusion chromatography | Tetramer | Monomer | Tetramer | Tetramer |
| Thermal denaturation temperature (Tm C) | 51.3 | 50.7 | 47.0 | 68.9 |
