(a) Hepatic triglyceride content (mg triglyceride/gram of liver) is increased in the FGF21-KO mice compared to WT on the MCD diet. (b) The lipid peroxidation product malondialdehyde is increased in the FGF21-KO mice on the MCD diet. (c) Collagen levels were higher in the liver of FGF21-KO mice (d) Hepatic mRNA expression of the pro-fibrotic genes are elevated in FGF21-KO mice fed MCD. (e) FGF21-KO mice have higher mRNA levels of the pro-inflammatory genes Cd68, Il1β, Mcp1, and Mip1a compared to WT mice. Data are expressed as mean ± SEM, with N = 6 mice per group. (*, P < 0.05; **, P < 0.01; *** P< 0.001).