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Effect of carbodiimide immobilization of E2-BSA complex within CG scaffold. (A) Fluorescent signal from E2-BSA immobilized within CG scaffolds. (B) Metabolic activity of epithelial cells in CG scaffolds treated with 1–1000 nM soluble E2 or scaffold-tethered 1–1000 nM E2 equivalent dose; results normalized to 0 nM E2 control. (C) Endogenous VEGF produced by epithelial cells in CG scaffolds treated with 1–1000 nM soluble E2 or scaffold-tethered 1–1000 nM E2 equivalent dose.
