Table 1.

Summarized results of spike and recovery tests

Sample	Added spike (pM)	Dilution rate	Expected (pM)	Observed (pM)	% recovery
					Average	Range
Culture media	50 pM	Neat	46	32	69.8	69–71
		1:2	46	40	85.8	85–87
		1:4	46	42	91.9	85–87
		1:8	46	44	95.1	93–98
Mouse plasma	200 pM	Neat	189	132	69.8	65–75
		1:5	201	180	89.6	65–75
		1:10	193	184	95.5	92–99
		1:20	190	197	103.5	103–104
	50 pM	Neat	47	29	61.8	58–66
		1:5	46	38	82.7	82–84
		1:10	46	40	88.7	88–89
		1:20	49	43	88.7	87–89

Values exceeding 80 % are presented