Mangiferin inhibited MAPK phosphorylation/activation. Expression of the phospho-ERK1/2 (1:1000), ERK1/2 (1:1000), phospho-JNK (1:2000), JNK (1:1000), phospho-p38 (1:1000), p38 (1:1000) and β-actin (1:2000) in the colon tissue was analysed by western blot (n=4). Quantification of the protein expression was performed by densitometric analysis of the blots. *p < 0.05, **P< 0.01 vs. DSS-treated group.