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. 2015 Sep 15;11(9):e1005160. doi: 10.1371/journal.ppat.1005160

Fig 5. rel Bbu regulates round body formation under starvation conditions in vitro.

Fig 5

Fluorescence microscopy of live B. burgdorferi wild-type (WT), rel Bbu mutant (rel Bbu -), and rel Bbu - pBS-rel Bbu strains grown in BSK + RS to late log phase (A-C) or shifted to RPMI for 2 days (D-F) and stained with WGA-Alexa Fluor 594. Scale bar = 10 μm. Scanning electron microscopy of rel Bbu - strains after shifting to RPMI for 2 days (G-J). Arrows indicate folded and disrupted membranes and arrowheads indicate membrane beading. Scale bar = 600 nm.