Table 4. Parameters extrapolated by the interpolation of the TF1 proliferation curves of human NGF wild type and mutants.
| Protein | Hmax (nm) a | C50 (ng/mL) b |
|---|---|---|
| hNGF WT | 2.5 ± 0.1 | 5.0 ± 1.3 |
| hNGF P61S | 2.0 ± 0.1 | 4.3 ± 1.3 |
| hNGF R100E | 1.5 ± 0.1 | 10.9 ± 2.1 |
| hNGF P61S R100E | 1.8 ± 0.1 | 8.1 ± 1.7 |
a Hmax is the maximum OD reached when the curve is saturated.
bC50 represents the concentration of hNGF determining half of the maximum effect on cell proliferation.
The values represent the average of the calculated Hmax and C50 parameters. The errors are standard deviations.