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. 2015 Sep 15;11(9):e1005159. doi: 10.1371/journal.ppat.1005159

Fig 4. Effect and localization of FLG2-4 on P. aeruginosa.

Fig 4

A-D: Electron microscopy of P. aeruginosa ATCC 33354 treated with FLG2-4 for 30 min (A-C) and untreated (D); E: Confocal laser scanning microscopy of FLG2-4-treated P. aeruginosa ATCC 33354, left panels: DRAQ5 staining, middle panels: FLG2-4 staining, left panels: merged images, upper panels: FLG2-4 treated bacteria, lower panels: untreated bacteria; F: Western Blot of fractionated, FLG2-4-treated, P. aeruginosa PAO1. Beneath, inner membrane fraction, localized by western blotting using antibodies directed against XcpY; SN: sample supernatant, CYT: cytoplasmic, IM: inner membrane, PP: periplasmic, OM: outer membrane enriched fractions, FLG2-4: FLG2-4 recombinant fragment. Approximate molecular mass in kDa is indicated on the left.