Table 1. Description of the TaqMan Gene Expression Assays used.
| Gene | Accession number | Exon | Amplicon length (bp) | Dye /Quencher | Assay number |
|---|---|---|---|---|---|
| HPRT1 (Reference gene) | NM_000194.2 | 1–2 | 72 | VIC / MGB | Hs01003267_m1 |
| GUSB (Reference gene) | NM_000181.3 | 8–9 | 96 | VIC / MGB | Hs00939627_m1 |
| PSMB6 (Reference gene) | NM_001270481.1 | 2–3 | 93 | VIC / MGB | Hs00382586_m1 |
| RPL13 (Reference gene) | NM_000977.3 | 6–6 | 137 | VIC / MGB | Hs00744303_s1 |
| TBP (Reference gene) | NM_001172085.1 | 2–3 | 91 | VIC / MGB | Hs00427620_m1 |
| MYC (Gene of interest) | NM_002467.4 | 1–2 | 87 | FAM / MGB | Hs00905030_m1 |
| Hif1α (Gene of interest) | NM_001530.3 | 1–2 | 62 | FAM / MGB | Hs00936371_m1 |
| CDX2 (Gene of interest) | NM_001265.4 | 2–3 | 81 | FAM / MGB | Hs01078080_m1 |
| HMOX1 (Gene of interest) | NM_002133.2 | 3–4 | 82 | FAM / MGB | Hs01110250_m1 |
| IL1β (Gene of interest) | NM_000576.2 | 3–4 | 91 | FAM / MGB | Hs01555410_m1 |