. Author manuscript; available in PMC: 2015 Sep 15.

Published in final edited form as: J Immunol Methods. 2014 May 22;408:78–88. doi: 10.1016/j.jim.2014.05.008

Table 2.

Assessment of assay accuracy and precision for detecting PA83 and PA63. Assay was repeated in duplicate 10 times (n) and for each the number of runs with a measurable results (n_m), expected mean (μM) the observed mean (μM), standard deviation (St. dev), coefficient of variation (CV), and percent error (% error).^a

Toxin	Expected mean (μM)	n _m /n	Observed mean (μM)	St. dev	CV (%)	% Error
PA83	0	3/10	0.0000028	0.0000023	81.7	ND
	0.000002	6/10	0.0000019	0.0000011	55.9	4.7
	0.00001	9/10	0.000014	0.000026	189.7	37.7
	0.001	10/10	0.0009	0.0002	20.8	11.8
	0.005	10/10	0.0041	0.0013	31.8	18.4
	0.01	10/10	0.012	0.005	41.2	23.5
	0.02	9/10	0.051	0.04	77.2	156.2
PA63	0	0/10	ND	ND	ND	ND
	0.000009	0/10	ND	ND	ND	ND
	0.00003	3/10	0.00012	0.00002	133.2	58.4
	0.008	10/10	0.0057	0.0005	8.2	23.4
	0.02	10/10	0.022	0.005	23.9	9.0
	0.05	10/10	0.055	0.018	32.7	10.5
	0.2	3/10	0.15	0.04	27.0	24.5

ND = not determined