Fig 4. Cell proliferation curves of CC-derived cell lines transfected with specific siRNAs against CDKN3 or scrambled siRNAs.

Cell lines were transfected with specific cyclin-dependent kinase inhibitor 3 (CDKN3) (red diamonds) or scrambled siRNAs (blue diamonds). Cells were harvested at 24, 48, 72, and 96 h after transfection. Cell proliferation was measured using the MTT method, and the plates were read with a spectrophotometer at 560 nm. The experiments were repeated two times by triplicate, and the figure shows the data of one experiment. Mean ± standard deviation of triplicates, growth trend line, and growth model equations are shown for CaSki (A), HeLa (B) and SiHa (C). Cell growth follows an exponential trend predicted by the equation y_t = y_{0 *} e^rx, where y_t = absorbance at specific time, x = incubation time in hours, y₀ = a constant absorbance at time = 0, and r = rate growth. In panel D, we calculated the percentage of cell-proliferation inhibition of CaSki, HeLa and SiHa cell lines at 24, 48, 72, and 96 h post-transfection. At each time point, the mean absorbance of triplicates of cells transfected with the scrambled siRNAs was set as 100%.