Fig. 5. Strong TCR stimulation induce naïve Th cells to express Tfh molecules.
(A) Bcl-6 expression by naïve Th cells transfected with the indicated siRNA and cultured for 3 days with anti-CD3 and CD28 ± sOX40L. Gated to FSChiSSChi activated cells. Mean ± s.e.m., n=3.
(B) Expression of the indicated markers by naïve Th cells activated for 4 days with the indicated number of anti-CD3 and anti-CD28-coated beads. Gated to FSChiSSChi activated cells. A representative result out of 3 independent experiments is shown.
