Figure 2.

(a) Bright field microscopy and CRM, and (b) targeted SIMS imaging and microspectroscopy of a 7-h P aeruginosa biofilm (a) (i) Bright field microscopy of a quinoline rich region found in 7-h static biofilms (ii) Raman image acquired from the circled region in (i) and integrated over 1338–1376 cm⁻¹ (iii) PC3 from the Raman image (iv) heat map showing the spatial distribution and magnitude of PC3 (b) SIMS imaging of regions of high AQ abundance Inset regions with variable color map intensity show that patterning is present for most AQs, however the intensity differences are most pronounced for the isomeric C₉-PQS/NQNO ion at m/z 28821 The mass spectrum is an average of four pixels from a representative high-intensity region of the TIC image (as indicated by the red arrow) The TIC images include values in the m/z 60–300 range, scaled from 0 to 2 counts, while the individual ion images are scaled as indicated by the color bar on the right.