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Conventional TEM images show that human corneal collagen is regularly spaced and arranged in cross-orientated layers (A). Standard TEM (B and C) and high pressure freezing TEM (D and E) demonstrate the presence of aggregates of loosely bundled smaller collagen filaments (highlighted by white arrows in D and E) that are aligned in a predominantly uniaxial orientation throughout the RHCIII and RHCIII-MPC hydrogels.
