Table 6. Correlation of sensitivity improvement, SSRCalc Hydrophobicity and MS images of peptides using CHCA+ADHB.^a) [Reproduced from refs. 31, 32 with Copyright permission of American Chemical Society.].

Peptides				Sensitivity improvement rate (fold)b)	MS imagingc)
No.	Name	SSRCalc Hydrophobicity	m/z (Av.)		CHCA+ADHB	CHCA
1	NF-κB inhibitor	54.8	2782.5	100
2	Melittin, honey bee	53.8	2847.5	10
3	β-Amyloid 1–42	51.1	4515.1	100
4	OVA-BIP hybrid peptide	50.2	2291.5	100
5	Humanin	50.0	2688.2	100
6	[Gly14]-Humanin	49.5	2658.2	10
7	Temporin A, amide	45.6	1397.8	10
8	MPGΔNLS	45.2	2767.2	10
9	β-Amyloid 22–42	42.4	2000.3	1
10	ACTH 18–39	37.9	2466.7	1
11	β-Amyloid 1–28	36.3	3263.5	1
12	β-Amyloid 1–11	13.5	1326.3	1
13	GPHRSTPESRAAV	10.6	1365.5	1
14	β-Conglycinin 165–178	5.2	1848.8	1

^a) Sensitivity improvement of fourteen peptides using CHCA+ADHB in positive-ion mode. ^b) The sensitivity improvement was calculated by dividing the detection limit using CHCA by that using CHCA+ADHB for differentially-analyzed peptides. ^c) MS images were created for each peptide in the fourteen 100 fmol peptide mixture analyses.