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. 2015 Sep 16;5:14142. doi: 10.1038/srep14142

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Copyright © 2015, Macmillan Publishers Limited

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EPC2-hTERT cells were transduced with lentiviral pLEX-ALDH2 or pLEX-ALDH2^MT vectors to establish EPC2-ALDH2 or EPC2-ALDH2^MT cells, respectively, which stably overexpressed wild-type or mutant ALDH2. As control cells, we transduced EPC2-hTERT cells with a lentiviral control vector bearing a FLAG-tag without an ALDH2 coding site. Data are presented as the mean ± SD. (a) Western blotting showing overproduction of FLAG-tagged ALDH2 protein (53.6 kDa) and non-FLAG-tagged ALDH2 (52.6 kDa) in EPC2-ALDH2 and EPC2-ALDH2^MT cells using antibodies to FLAG M2 and ALDH2; β-actin served as a loading control. (b) N²-ethylidene-dG levels in EPC2-ALDH2, EPC2-ALDH2^MT, and control cells treated with 0 or 0.2 mM acetaldehyde for 72 h (**p < 0.01, *p < 0.05 between the indicated groups; n = 3 in each group; n.s., not significant).