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. 2015 Sep 16;5:14194. doi: 10.1038/srep14194

Figure 2. Basic characterizations of the responsive probe TOB-Eu3+ and its on-state compound TOHB-Eu3+.

Figure 2

(a) Time-gated excitation (λem = 608 nm) and emission (λex = 330 nm) spectra of TOB-Eu3+ (0.5 μM) in the presence of different concentrations of vitamin C in 1:1 ethanol-0.05 M Tris-HCl buffer of pH 7.5. (b) UV-vis absorption spectra of TOB-Eu3+ (10 μM, black) and TOHB-Eu3+ (10 μM, red) in 1:1 ethanol-0.05 M Tris-HCl buffer of pH 7.5. (c) Effects of pH on the luminescence intensities of TOB-Eu3+ (0.5 μM, black) and TOHB-Eu3+ (0.5 μM, red) in solutions of 0.05 M Tris-HCl/ethanol (v/v = 1:1) with different pH values. (d) Calibration curve for vitamin C measured on Perkin Elmer Victor 1420 multilabel counter.