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. 2015 Sep 16;5:14194. doi: 10.1038/srep14194

Figure 4. Time-gated luminescence imaging and real-time quantifications of the extracellular vitamin C uptake and intracellular production of vitamin C in living cells.

Figure 4

(a) Time-gated luminescence microscopy images of HepG2 cells loaded with 1.0 mM vitamin C at different loading times and followed by incubation with 20 μM TOB-Eu3+ for 1 h. Scale bar: 50 μm. (b) Time-gated luminescence intensities of HepG2 (•) and HeLa (■) cells loaded with 1.0 mM vitamin C for various incubation times and followed by incubation with 20 μM TOB-Eu3+ for 1 h. (c) Time-gated luminescence intensity of HepG2 cells loaded with 1.0 mM AA (■) or 1.0 mM DHA (•) for various incubation times and followed by incubation with 20 μM TOB-Eu3+.