Figure 3.

Change in stiffness and fluidity of K562 leukemia cells after drug treatment. (a) Scatter plots of entry times versus applied pressure for control cells and for cells treated with Mg²⁺Ca²⁺-ions (mgca), 5-AZA-2′-deoxycytidine (AZA), cytochalasin D (cytoD), glutaraldehyde (ga), nocodazole (noc), paclitaxel (pax), and acrylamide (acry); n > 2000 cells for each measurement. (Solid markers) Geometric mean of ∼200–400 cells binned according to pressure. (Solid lines) Fit of Eq. 3 to the binned data. Fit to control data is shown in all other plots for comparison (dashed line). (b) Population average of cell stiffness (top) and cell fluidity (bottom) for different drug treatments. Error bars represent SDs calculated by bootstrapping. (Asterisks) Significant differences with p < 0.0005. To see this figure in color, go online.