Figure 4.

Effect of lamin A-overexpression on cell mechanics. (a) Scatter plots of entry times versus applied pressure for control cells and for GFP-lamin A-induced K562 cells. (Solid markers) Geometric mean of ∼300–500 cells binned according to pressure. (Solid lines) Fit of Eq. 3 to the binned data. Fit to control data is shown for comparison in the LaA+ plot (dashed line). (b) Population average of cell stiffness (top) and cell fluidity (bottom) for lamin A-overexpressing cells differ significantly (p < 0.0005 as indicated by asterisks) from control. Error bars represent SDs calculated by bootstrapping. To see this figure in color, go online.