Table 3.
Property | Ficoll-70 | Dextran-10 | Sucrose |
---|---|---|---|
Structure | |||
Interdomain distance | ↓ | ↓ | ↓ |
Residue conformation | NC | NC | NC |
Diffusion | |||
Rotational tumbling | ↓ | ↓ | ↓ |
Translational diffusion | ↓ | ↓ | ↓ |
Steady-state affinity | |||
Ca2+ affinity | NC | NC | ↓ |
CaMKI peptide affinity | NC | NC | NC |
Kinetic rates | |||
CaMKI association | ↓ | ↓ | ↓ |
Conformational rearrangements for binding | ↑ | ↑ | ↑ |
CaMKI dissociation | ↓ | ↓ | ↓ |
The effects of ficoll-70, dextran-10, and sucrose are summarized for each property of CaM investigated. (Arrows, increases and decreases; NC, no change.)