Fig. 4.

Apoptosis induction in non-stimulated Jurkat-Tat101 cells. a Percentage of Jurkat-Tat72, Jurkat-Tat101 and control cells showing an apoptotic phenotype from three independent experiments analyzed by confocal microscopy. b Representative images of living and apoptotic cells. Cells were fixed and the nuclei were stained with Dapi. c Caspase-3/-7 activation was measured by a chemiluminiscence-based test in Jurkat-Tat72, Jurkat-Tat101 and control cells under basal conditions. Relative luciferase units (RLUs) after total protein normalization are shown. d Percentage of apoptosis committed cells expressing external phosphatidylserine. The graph shows the cytometry analysis of Annexin-V-PE stained cells. e Cytometry analysis of doubly stained cells for Annexin-V-PE and DCF-DA-FITC. Graph on the left shows the percentage of double stained cells. Graph on the right shows G-mean of green fluorescence intensity in double stained cells. All graphs show media and SEM from at least three independent experiments. Kruskal–Wallis test with Dunn’s multiple comparison post hoc analysis was performed for statistical analysis (*p < 0.05, **p < 0.001)