Table 1.
Selection of mitochondrial-related proteins which showed at least a ±2.0-fold deregulation in Jurkat-Tat101 versus control cells
| Gene | Protein | Tat72 vs TetOff | Tat101 vs TetOff | Biological process | Peptide sequence | X-corr |
|---|---|---|---|---|---|---|
| PKM2 | Pyruvate kinase isozymes M1/M2 | 6.5 | 36.8 | Glycolysis | APIIAVTRNPQTAR EAEAAIYHLQLFEELRR FGVEQDVDMVFASFIR GSGTAEVELKK IYVDDGLISLQVK KGVNLPGAAVDLPAVSEKDIQDLK LDIDSPPITAR RFDEILEASDGIMVAR SVETLKEMIK TATESFASDPILYRPVAVALDTK TATESFASDPILYRPVAVALDTKGPEIR |
2.65 1.81 2.90 2.06 1.88 3.72 2.01 3.16 1.96 2.80 3.18 |
| LDHB | l-lactate dehydrogenase (B chain) | −0.9 | 10.4 | Glycolysis | IVADKDYSVTANSK LKDDEVAQLKK SADTLWDIQK SADTLWDIQKDLKDL |
2.07 2.26 2.05 2.08 |
| SH3BGRL3 | SH3 domain-binding glutamic acid-rich-like protein 3 | 9.9 | 8.1 | Oxidative stress | IQYQLVDISQDNALRDEMR VYSTSVTGSR |
3.12 2.48 |
| PRDX1 | Peroxiredoxin-1 | 7.9 | 7.8 | Oxidative stress | QGGLGPMNIPLVSDPKR QITVNDLPVGR TIAQDYGVLKADEGISFR |
1.78 2.06 2.95 |
| PDIA4 | Protein disulfide-isomerase A4 | 0.7 | 5.2 | Oxidative stress | FDVSGYPTIK FDVSGYPTLK IDATSASVLASR MDATANDVPSDR VDATAETDLAKR |
1.98 1.98 2.51 1.75 1.83 |
| TKT | Transketolase | −1.2 | 4.9 | Energy metabolism | ILATPPQEDAPSVDIANIR KAYGQALAK MFGIDRDAIAQAVR |
1.80 2.29 2.71 |
| TXNDC17 | Thioredoxin domain-containing protein 17 | 0.9 | 2.8 | Oxidative stress | YEEVSVSGFEEFHR | 1.77 |
| SOD1 | Superoxide dismutase | 0.4 | 3.3 | Oxidative stress | TLVVHEKADDLGK TLVVHEKADDLGKGGNEESTK |
1.76 3.07 |
| ALDOA | Fructose-bisphoshate aldolase A | 8.9 | 2.7 | Glycolysis | AAQEEYVKR GILAADESTGSIAK GILAADESTGSIAKR IGEHTPSALAIMENANVLAR IVAPGKGILAADESTGSIAK IVAPGKGILAADESTGSIAKR LQSIGTENTEENR |
1.88 3.03 2.61 2.69 3.80 3.91 2.41 |
| P4HB | Protein disulfide-isomerase | 1.5 | 2.5 | Oxidative stress | MDSTANEVEAVKVHSFPTLK | 2.02 |
| GYS1 | Glycogen synthase, muscle | 0.9 | 2.5 | Glycogenesis | LSDLLDWK TQVELLEAPTPALKR VGGIYTVLQTK |
1.79 1.94 1.87 |
| TPM4 | Tropomyosin alpha-4 | 9.9 | 2.2 | Oxidative stress | KIQALQQQADEAEDR | 2.13 |
| TALDO1 | Transaldolase | 0.6 | 2.2 | Energy metabolism | WLHNEDQMAVEK | 2.15 |
| PEBP1 | Phosphatidylethanolamine-binding protein | 0.0 | 2.2 | Oxidative stress | LYEQLSGK WSGPLSLQEVDEQPQHPLHVTYAGAA |
1.79 2.68 |
| PARK7 | Protein DJ-1 | 1.8 | −2.0 | Autophagy Fusion Morphogenesis |
GAEEMETVIPVDVMR GAEEMETVIPVDVMRR |
2.08 2.00 |
| PGK1 | Phosphoglycerate kinase 1 | 1.3 | −2.7 | Glycolysis | AHSSMVGVNLPQK LGDVYVNDAFGTAHR VDFNVPMKNNQITNNQR VLPGVDALSNI |
1.81 2.60 2.00 1.96 |
| TPI1 | Triosephosphate isomerase isoform 1 | −5.3 | −3.0 | Glycolysis gluconeogenesis | SNVSDAVAQSTR VVLAYEPVWAIGTGK |
2.95 2.65 |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | −10.8 | −11.4 | Glycolysis | GALQNIIPASTGAAK IKWGDAGAEYVVESTGVFTTMEK LISWYDNEFGYSNR VGVNGFGR VVDLMAHMASKE WGDAGAEYVVESTGVFTTMEK |
1.81 2.17 3.07 2.09 2.87 2.53 |
| PDIA3 | Protein disulfide-isomerase A3 | −15.0 | −11.9 | Oxidative stress | DGEEAGAYDGPR EATNPPVIQEEKPK ELSDFISYLQR GFPTIYFSPANK IFRDGEEAGAYDGPR KYEGGRELSDFISYLQR LSKDPNIVIAK MDATANDVPSPYEVR QAGPASVPLR QAGPASVPLRTEEEFKK RLAPEYEAAATR TADGIVSHLKK YGVSGYPTLK |
2.09 2.30 2.43 2.34 3.09 2.91 2.14 2.62 2.59 1.82 2.68 2.68 2.05 |
Proteins were detected by mass spectrometry in total protein extracts. All peptides detected showed 95 % probability for protein expression and a minimum cross-correlation (X-Corr) value of 1.75