Table 3.
Selection of proteins deregulated at least ±2.0-fold in Jurkat-Tat101 versus control cells
| Gene | Protein | Tat72 vs TetOff | Tat101 vs TetOff | Biological process | Peptide sequence | X-corr |
|---|---|---|---|---|---|---|
| CORO1A | Coronin-1A | 9.3 | 36.8 | Actin cytoskeleton organization | KLQATVQELQK | 2.93 |
| MYL12B | Myosin regulatory light chain 12B | 3.0 | 13.2 | Regulation of cell shape | ELLTTMGDRFTDEEVDELYR GNFNYIEFTR |
1.75 2.60 |
| EZR | Ezrin | 11.2 | 11.9 | Actin filament bundle assembly | QLLTLSSELSQAR QLLTLSSELSQARDENKR |
1.79 2.06 |
| SEPT2 | Septin-2 | 0.0 | 5.2 | Actin cytoskeleton organization | TMLITHMQDLQEVTQDLHYENFR | 2.29 |
| CFL1 | Cofilin-1 | 0.9 | 5.2 | small GTPase signal transduction | LGGSAVISLEGKPL | 2.33 |
| ACTN4 | Actinin-alpha-4 | 0.0 | 4.4 | Actin filament bundle assembly | MAPYQGPDAVPGALDYK GISQEQMQEFR |
2.11 2.11 |
| TUBB2C | Tubulin beta-2C | 6.4 | 4.4 | Microtubule organization | INVYYNEATGGK | 1.75 |
| SLC9A3R1 | Na/H exchange regulatory cofactor (NHE-RF1) | 3.1 | 3.5 | Actin cytoskeleton organization | SVDPDSPAEASGLR SVDPDSPAEASGLRAQDR |
1.95 1.33 |
| PPP1R12A | Protein phosphatase 1 regulatory subunit 12A | 5.2 | 2.8 | Actin cytoskeleton organization | NKETLIIEPEKNASR | 2.12 |
| TPM4 | Tropomyosin alpha-4 chain | 9.9 | 2.2 | Actin cytoskeleton organization | KIQALQQQADEAEDR | 1.65 2.13 |
| TBCA | Tubulin-specific chaperone A | 2.1 | 2.2 | Tubulin assembly | LVLDSVKLEA RLEAAYLDLQR |
1.78 1.84 |
| VIM | Vimentin | 3.9 | 2.2 | Cytoskeleton organization | FADLSEAANR | 1.75 |
| GDI2 | Rab GDP dissociation inhibitor beta | 0.0 | 2.0 | small GTPase signal transduction | MTGSEFDFEEMKR | 1.78 |
| TUBA1B | Tubulin alpha-1B | 3.7 | −3.4 | Microtubule cytoskeleton organization | TIGGGDDSFNTFFSETGAGK DVNAAIATIK |
1.91 2.00 |
| TUBB | Tubulin beta | −1.3 | −6.9 | Microtubule cytoskeleton organization | FWEVISDEHGIDPTGTYHGDSDLQLDR AILVDLEPGTMDSVR EVDEQMLNVQNK MSMKEVDEQMLNVQNK |
2.16 2.20 2.74 4.08 |
| MYH10 | Myosin-10 | −2.3 | −8.5 | Actin filament-based movement | ELDDATEANEGLSREVSTLKNR QLEEAEEEATRANASR ELDDATEANEGLSR |
1.99 2.38 2.78 |
| ARHGDIB | Rho GDP-dissociation inhibitor 2 | −2.6 | −10.0 | small GTPase signal transduction | TLLGDGPVVTDPKAPNVVVTR | 1.97 |
| STMN1 | Stathmin | −28.5 | −30.5 | Microtubule depolymerization | RAsGQAFELILsPR | 2.19 |
| TMSL3 | Thymosin beta-4-like protein 3 | −28.8 | −33.4 | Actin cytoskeleton organization | NPLPSKETIEQEKQAGES NPLPSKETIEQEK TETQEKNPLPSKETIEQEK |
1.80 2.31 2.65 |
| ACTB | Actin cytoplasmic | −15.0 | −43.7 | Actin cytoskeleton organization | DSYVGDEAQSK DLYANTVLSGGTTMYPGIADR DSYVGDEAQSKR HQGVMVGMGQK AVFPSIVGRPR TTGIVMDSGDGVTHTVPIYEGYALPHAI QEYDESGPSIVHR HQGVMVGMGQKDSYVGDEAQSK IWHHTFYNELR MQKEITALAPSTMK |
1.83 1.87 1.88 1.89 1.98 2.15 2.37 2.81 3.31 3.52 |
Only proteins related to actin and tubulin cytoskeleton networks were selected. All peptides selected showed 95 % probability for protein expression and a minimum X-corr of 1.75