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. 2015 Sep 15;17(1):253. doi: 10.1186/s13075-015-0763-6

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© Miyazaki et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effect of recombinant human silent mating type information regulator 2 homolog 1 (rhSIRT1) on the number of human nucleus pulposus (NP) cells. a Morphological appearance of NP cells from intervertebral discs (IVDs) of Pfirrmann grade II and IV after 14 days of treatment with 10 μM rhSIRT1 or 5 mM 3-methyladenine (3-MA) under normal nutrition (N) conditions with 10 % (v/v) fetal bovine serum (FBS) or low nutrition (LN) conditions with 1 % (v/v) FBS (bars, 250 μm). b Changes in the number of NP cells from IVDs of Pfirrmann grades II and IV after 0, 3, 7, and 14 days of treatment. Data are expressed as the mean ± standard deviation (n = 5). *P < 0.05; ^† P < 0.01, three-way analysis of variance with the Tukey–Kramer post hoc test