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Pma1-GFP does accumulate in a compartment enriched for an ER marker. (A) Pma1-GFP is retained in ER subcompartments in Δglo3Δerg3 cells. Cells co-expressing Pma1-GFP and the ER marker Sec63-RFP were analyzed. (B) The Pma1 accumulations in Δglo3Δerg3 cells are not positive for the Golgi marker Anp1. Early- to mid-log phase grown cells expressing Pma1-GFP and Anp1-mCherry were analyzed by microscopy. Scale bars represents 5 µm.
