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. 2015 May 15;4(7):873–884. doi: 10.1242/bio.011734

Fig. 3.

Fig. 3.

BAZ1B regulates γH2AX-MDC1 signaling in early pachytene spermatocytes. (A) The percentage of each stage of meiotic prophase as judged by SYCP3 immunostaining from two independent Baz1bctrl or Baz1bcKO mice. (B,C) Immunostaining of chromosome spreads with γH2AX and SYCP3. Scale bars, 10 µm unless otherwise indicated. The areas shown with white dotted squares are magnified in the bottom panels. (D) Scoring of population of cells with normal γH2AX domain on the sex chromosomes from two independent Baz1bctrl or Baz1bcKO mice. (E) Scoring of population of cells with ectopic γH2AX signaling on synapsed autosomal regions from two independent Baz1bctrl or Baz1bcKO mice. (F) Immunostaining of chromosome spreads with MDC1 and SYCP3. Arrows indicate ectopic γH2AX-MDC1 signaling on synapsed autosomal regions. Scale bars, 10 µm. *P<0.05, unpaired t-test.