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. 2015 May 15;4(7):873–884. doi: 10.1242/bio.011734

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 7. — BAZ1B-independent localization of pTyr142 in meiotic spermatocytes and in round spermatids. (A-C,D,F) Immunostaining of chromosome spreads of (A) leptotene, (B) zygotene, (C,D) pachytene (E) mid diplotene and (F) late diplotene spermatocytes with pTyr142 and γH2AX. (D,G) Immunostaining of slides that preserve the relative 3D chromatin structure of (D) pachytene and (G) round spermatid spermatogenic cells (3D slides) stained with pTyr142 and γH2AX. Single Z-sections were shown. Scale bars, 10 µm. Dotted circles indicate the area of the sex chromosomes. (H) Models of the mode of two phosphorylation sites of H2AX during meiosis.