Fig. 8.

MDC1, but not RNF8, is required for dephosphorylation of pTyr142 on the sex chromosomes in pachytene spermatocytes. (A,C) Immunostaining of chromosome spreads with pTyr142 and γH2AX. Scale bars, 10 µm. Dotted circles indicate the area of the sex chromosomes. (B) Models of MDC1-dependent regulation of two phosphorylation sites of H2AX during meiosis. (D) Immunostaining of slides that preserve the relative 3D chromatin structure of spermatogenic cells (3D slides) with MCPH1 and γH2AX. Scale bars, 10 µm. Dotted circles indicate the area of the sex chromosomes.