Fig. 6.

Embryonic 10T1/2 fibroblasts require cell-matrix adhesion, but not a stiff substrate to complete cytokinesis. (A) Mitotic 10T1/2 cells were collected and replated onto either fibronectin-coated coverslips (adhesion) or poly-HEMA coated dishes (suspension) at 37°C for 5 h. Cells were stained for α-tubulin and nuclei. Plotted is the mean percentage of binucleated cells±s.e.m. from three independent experiments in which more than one hundred cells were counted per condition for each experiment (***P<0.0005). (B) Mitotic 10T1/2 cells were collected by the shake-off method and replated onto fibronectin-coated hard and soft substrates in DMEM with 10% FBS for 5 h at 37°C and then fixed and stained for α-tubulin, lamin-B1, and nuclei. Plotted is the mean percentage of binucleated cells on hard and soft substrates±s.e.m. from three independent experiments in which one hundred cells were counted per condition for each experiment, ns (non-significant).