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. 2015 May 15;4(7):852–857. doi: 10.1242/bio.012344

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — The increase in ecdysone signaling is delayed in CTCF knockdown larvae. (A) The percentages of larvae of the control (phm>w) and CTCF RNAi (phm>CTCF^RNAi) genotypes that underwent pupariation were plotted relative to the time in days after egg laying. Error bars show s.d. of three independent experiments. (B) Transcriptional levels of Halloween genes in CTCF RNAi at the end of larval development as fold changes relative to control (phm>w 120 h). Error bars show s.e.m. of at least three independent experiments. ***P<0.001, **P<0.01, *P<0.1 with Student's t-test. (C) Temporal profile of ecdysone signaling gene E74B transcriptional induction in control and CTCF RNAi larvae. Error bars show s.e.m. of three independent experiments.