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. 2015 May 15;4(7):852–857. doi: 10.1242/bio.012344

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Both, 20-hydroxyecdysone (20E) and cholesterol (C) are required to rescue CTCF knockdown phenotypes. (A) Control (phm>w) and CTCF RNAi (phm>CTCF^RNAi) larvae were fed food containing EtOH, 20E or/and C and the percentages of larvae of the indicated genotypes that underwent pupariation were plotted relative to the time in days after egg laying. Error bars show s.d. of three independent experiments. (B) Lipid accumulation in control and CTCF RNAi ecdysone-producing cells. Single plane confocal micrographs showing lipid droplets in PG cells stained with Oil Red O (either in black and white, upper panels, or in red, lower panels) of 120 h AEL clear-gut phm>w control larvae (left panels), 120 h AEL blue-gut phm>CTCF^RNAi larvae (middle panels) and 150 h AEL clear-gut phm>CTCF^RNAi larvae (right panels). DNA was labeled with DAPI (blue).